Onder andere met: Robert De Niro (Jack Byrnes), Ben Stiller (Greg 'Gaylord' Focker), Owen Wilson (Kevin Rawley), Dustin Hoffman (Bernie Focker), Barbra Streisand (Roz Focker), Blythe Danner (Dina Byrnes), Teri Polo (Pam Focker), Jessica Alba (Andi Garcia), Laura Dern (Prudence), Kevin Hart (Nurse Louis), Daisy Tahan (Samantha Focker), Colin Baiocchi (Henry Focker), Thomas McCarthy (Dr. Bob), Harvey Keitel (Randy Weir), Yul Vazquez (Junior), Jack Axelrod (Chappy), Olga Fonda (Svetlana), Laksh Singh (Dr. Patel), Clent Bowers (Mr. Androvsky)