Onder andere met: Tobey Maguire (Spider-Man/Peter Parker), Kirsten Dunst (Mary Jane Watson), James Franco (New Goblin/Harry Osborn), Thomas Haden Church (Sandman/Flint Marko), Topher Grace (Venom/Eddie Brock), Bryce Dallas Howard (Gwen Stacy), Rosemary Harris (May Parker), J.K. Simmons (J. Jonah Jameson), James Cromwell (Captain Stacy), Theresa Russell (Emma Marko), Dylan Baker (Dr. Curt Connors), Bill Nunn (Joseph `Robbie` Robertson), Bruce Campbell (Maître d’), Elizabeth Banks (Miss Brant), Ted Raimi (Hoffman)