Onder andere met: Nathan Fillion (Mal), Gina Torres (Zoe), Alan Tudyk (Wash), Morena Baccarin (Inara), Adam Baldwin (Jayne), Jewel Staite (Kaylee), Sean Maher (Simon), Summer Glau (River), Ron Glass (Shepherd Book), Chiwetel Ejiofor (The Operative), David Krumholtz (Mr. Universe), Michael Hitchcock (Dr. Mathias), Sarah Paulson (Dr. Caron), Yan Feldman (Mingo), Rafael Feldman (Fanty), Nectar Rose (Lenore)